AFP and neoplasm: First, female and male BALB/c mice were injected with 5 × 105 Hepa1-6 or H22 tumor cells s.c. in the left flank followed by vaccination s.c. in the right flank with PBS, AFP-P, HSP70-P or HSP70-P/AFP-P (100 μl/mouse) on days 3, day 10 and day 17.