Regarding clinical data, 5 of 22 patients included in this work died during this study, of which four(patient 4, 6, 8 and 9) had CTCs isolated with anti-EGFR (3, 2, 9 and 4 CTCs, respectively) while patient 11, who had no CTCs in the EGFR fraction, died from a tumour-independent cause. This evidence concerns the gene EGFR and neoplasm.