Our results, further confirmed by the N‐acetyl cysteine (NAC) and specific inhibitors’ administration showed erinacine A's involvement in the PI3K/mTOR/p70S6K pathway and anti‐invasion, thereby showing COFL1 and PROF1, which potentially involved in anticancer mechanisms of erinacine A in the human HCT‐116 and DLD‐1 cells, previously had been implicated in the cancer‐related actin depolymerization pathway and CRC therapy 17. Here, MTOR is linked to colorectal carcinoma.