In our aim to illustrate the molecular mechanisms underlying erinacine A–induced ROS production and the activation of the PI3K/mTOR/p70S6K signalling pathway in HCT‐116 CRC cells, we found that the protein profiles offered information leading to the discovery of specific biomarkers and the mechanism of H. erinaceus mycelium. Here, RPS6KB1 is linked to colorectal carcinoma.