Quantification of confocal images, as pixel sum of each antibody labeling in GFAP‐positive pixels, showed an increased expression of these protein levels in hippocampal astrocytes of 18‐month‐old 3xTg‐AD mice (integrin β1: 158 ± 32% and NOX2: 147 ± 27%), as well as GFAP protein levels (334 ± 36%, n = 3), compared to age‐matched non‐Tg mice (100%). Here, GFAP is linked to Alzheimer disease.