We also confirmed that upon challenge with a lethal dose of trypomastigotes, the mice co-immunized with the gp83 neutralizing epitope, and the ASP-M epitope capsid-incorporated vector displayed a significant reduction in parasitemia, improvement of their survival rate by eliciting neutralizing antibodies and CD8+ T cells capable of stimulating CD107a, TNFα, and IFNγ in response to the ASP-M epitope. Here, CD8A is linked to parasitic infectious disease.