When the phosphorylation levels of Akt in the whole liver were measured in the fasting and fed states under the NC and HF diet conditions, the Akt phosphorylation levels were found to be not significantly different in the mice on NC or HF diet in the fed state, whereas in the fasting state, the phosphorylation level was significantly increased in the mice on a HF diet as compared with those on NC diet (Supplementary Figs 6 and 7). Here, AKT1 is linked to hydrops fetalis.