To examine whether CASP1 modulates ODBP in NLRP3 inflammasome-dependent manner, we obtained Nlrp3−/− mice and found that, while the mice lacking NLRP3 had less weight gain with HFD than WT mice (Supplementary Fig. 3), NLRP3 deficiency had no significant impact on tumour growth in DIO or normal-weight mice (Fig. 2c). This evidence concerns the gene NLRP3 and neoplasm.