Quantification of relative mRNA expression (Figure 5B, middle) as well as ELISA performed on cell-free supernatants (Figure 5B, right), revealed that downregulation of SK1 (Figure 5B, left) is associated with a significant decrease in TGF-β1 levels in melanoma cells, this phenomenom being amplified by SKI-I. Here, TGFB1 is linked to melanoma.