Next, HEK293T cells and HCC cell lines HepG2 and HuH-7 were transfected with eGFP-PRMT5 (green) and mCherry-CDK4 (red), and yellow signals indicating their co-localization were detected both in cytoplasm and nucleus in the merged images (Figure 4F). This evidence concerns the gene CDK4 and hepatocellular carcinoma.