Compared with the T2DM nonintervening group, the expression of cAMP-1 and PKA was increased, but the expressions of MLCK and PKC were decreased in the early- and mid-phase DDD-intervening groups (P < 0.01); the expressions of PKA, MLCK, PKC, and cAMP-1 in the early-phase DDD-intervening group were better than those of the late-phase DDD-intervening group (P < 0.05). Here, MYLK is linked to type 2 diabetes mellitus.