To determine whether the mutations placed within the Grasp65-7A protein sequence prevented its phosphorylation in the context of HCMV infection, stable cell lines expressing either the Grasp65wt-GFP or Grasp65-7A–GFP mutant protein were infected with HCMV at an MOI of 1, and cell lysates were assayed by immunoblotting using an anti-GFP antibody. The gene discussed is GORASP1; the disease is cytomegalovirus infection.