GAD1 and major depressive disorder: The studies from depression subjects indicate the low GABAergic tone in the central nervous system.24, 25, 26, 27, 28, 29, 30, 64, 65 The enhancers of GABAA receptors have been used as antidepressants; however, there is controversy in therapeutic outcomes.56, 66, 67, 68, 69, 70 This discrepancy may be because of the increased responsiveness or density of GABAA receptors.19 On the other hand, GABA releases and reuptakes in presynaptic terminals' decrease by upregulating miRNAs and downregulating mRNAs/proteins related to GAD-67, VGAT and GAT-3 in CUMS-induced depression mice (Figures 2 and 5).