Figure 4c illustrates the levels of such mRNAs quantified using high-throughput sequencing, where GAD1, VGAT and GAT-3 are significantly lower in the medial prefrontal cortices from CUMS-induced depression mice than those from control mice. Figure 4d illustrates the levels of such mRNAs quantified by their qRT-PCR, in which GAD1, VGAT and GAT-3 are significantly lower in the medial prefrontal cortices from CUMS-induced depression mice than in those from control mice. This evidence concerns the gene SLC32A1 and depressive symptom measurement.