Figure 4a, b shows western blot analyses about the levels of proteins of GAD-67, VGAT and GAT-3 that are harvested from the medial prefrontal cortices of CUMS-induced depression mice and controls. The densities of GAD-67, VGAT and GAT-3 bands appear lower in CUMS-induced depression mice, compared with those in controls (Figure 4a). Statistical analyses in Figure 4b demonstrate that the levels of GAD-67, VGAT and GAT-3 are significantly lower in the medial prefrontal cortices from CUMS-induced depression mice than those in control mice. The gene discussed is GAD1; the disease is depressive disorder.