Cellular senescence, a state of irreversible growth arrest, which plays a major role in aging and cancer is often accompanied by morphological changes such as cells becoming large, flat and multinucleated.19 Since we observed that growth arrest was accompanied by the presence of large, flat and vacuolated cells in KDM3A knockdown cells, we stained for senescence-associated β-galactosidase activity, a hallmark of cellular senescence. This evidence concerns the gene KDM3A and cancer.