AKT1 and breast cancer: Interestingly, while the canonical growth factor signalling molecules in breast cancer such as AKT and ERK were not activated with TLK2 overexpression, markedly increased phosphorylation of SRC on Y416 in the activation loop of its kinase domain, as well as modest increased activating phosphorylation of FAK and p38 were observed in the T47D cells overexpressing TLK2 (Fig. 3e).