To systematically profile the cell signalling changes after TLK2 inhibition, we performed reverse phase protein array (RPPA) analysis of the MCF7 cells treated with TLK2 siRNAs as well as the xenograft tumours harvested after two weeks of TLK2 inhibition, using 200 validated antibodies against an array of key signalling molecules in cancer. The gene discussed is TLK2; the disease is cancer.