Herein, we applied our established nanopore film based assay to investigate serum hepcidin-25 levels in a cohort of patients with CHB, HBV-related cirrhosis or HBV-related cirrhosis with hepatocellular carcinoma (HBV-HCC), in order to better understand the associations between hepcidin and HBV-related diseases. This evidence concerns the gene HAMP and hepatocellular carcinoma.