Results showed that GATA6 had a trend of lower expression in BM742401-methylated cell lines (MEC1, I83-E95 and WAC3CD5, HG3) than BM742401-unmethylated cell lines (MEC2, 232B4, and CLL-AAT) (P = 0.14) (Supplementary Figure S2), as indicated by a higher ΔCt (Ct GATA6 − Ct GAPDH) in BM742401-methylated than BM742401-unmethylated CLL cell lines. This evidence concerns the gene GAPDH and B-cell chronic lymphocytic leukemia.