In conclusion, our study reveals that TRIM24 mRNA and protein are reduced in ESCC tissues and the reduced TRIM24 protein is associated with LNM, advanced TNM stage and post-operative recurrence and metastasis; more important, TRIM24 protein level has been proven to be an independent prognostic factor for OS in ESCC patients. The gene discussed is TRIM24; the disease is esophageal squamous cell carcinoma.