To verify whether the expression levels of SMC4 differed between lung ADC samples and normal lung samples, we first performed a real-time RT-PCR assay of paired lung ADC tissues and adjacent normal lung tissues and found that the expression level of SMC4 mRNA is 1.8-fold higher in 43 lung ADC tissues compared with their adjacent normal lung tissues (Two-tailed paired t-test, p = 0.0012; Fig. 3A). The gene discussed is SMC4; the disease is medical procedure.