MLKL and cholestasis: Finally, p-MLKL colocalized with thioflavin T staining (P<0.05; Figure 1b), indicating that MLKL was phosphorylated in the presence of amyloid aggregates, characteristic of necrosome complexes.6 Altogether, our results indicate that necroptosis is activated in liver parenchymal cells of PBC patients, likely contributing to cholestasis and liver injury.