SMAD4 and gastric cancer: Stable over-expression of miR-558 in gastric cancer cells led to enhanced binding of AGO1 and decreased enrichment of Smad4, which was inhibited by knockdown of AGO1 (Figure 2h), without changes in the enrichment of epigenetic markers histone H3 lysine 9 dimethylation (H3K9me2), histone H3 lysine 27 trimethylation (H3K27me3) or histone H3 lysine 4 trimethylation (H3K4me3) on HPSE promoter (Figure 2h).