Like embryonic stem cells (ESC), several transcriptional factors regulating CSCs self-renewals and pluripotent properties were found in various cancers.18, 19 They are Oct4, Sox2, Klf4 and Nano and so on whose upregulation in cancer cells are usually related to stemness maintaining, tumor local invasion and distant metastasis.20 To bring insight into the roles of miR-612, mRNA and protein levels of above four transcriptional factors were first detected in miR-612-i HepG2 and miR-612-o HCCLM3 cells, respectively. This evidence concerns the gene POU5F1 and cancer.