Previous studies of alternative biomarkers from analyses of QFT supernatants [8–11] have found that unstimulated or TB antigen stimulated levels of various markers (EGF, MIP-1β, TGF-α, IL-1α, sCD40L, VEGF, IFN-α2, IL-1ra, IP-10, IL-2, IL-15 and MCP-1) may have potential for differentiating between active TB and LTBI. This evidence concerns the gene CD40LG and tuberculosis.