The Early Group was administrated at 4 weeks after DEN treatment, all eight mice injected with luciferase shRNA vector as controls developed tumor nodules in livers when sacrificed at 18 weeks, whereas the other eight mice injected with adenovirus for Dlk1 knockdown showed the significant reduction of number and size of tumor nodules as compared to the controls (Figure 4A–4C). The gene discussed is DLK1; the disease is neoplasm.