To confirm this T helper phenotype, we performed intracellular cytokine staining (ICS) for Th1 (IFN-γ), Th2 (IL-13), Th17 (IL-17A) and Treg (IL-10) associated cytokines in polyclonally stimulated lung leukocytes on day 7 post-infection. Here, IFNG is linked to infection.