KRT8 and colorectal cancer: Figure 2e clearly shows that HCT116, DLD1, and Caco2 cells strongly expressed keratins 8, 18, and 19, whereas RKO cells did not. These results support the hypothesis, proposed by in vivo experiments, that CK1α and FAM83H are localized to nuclear speckles in colorectal cancer cells without an intact keratin cytoskeleton.