In addition, we investigated Helios gene expression by RT-PCR analysis in non-hematopoietic human cells, for example the hepatic cancer cell line HEPG2 and SK-HEP1, breast cancer cell lines MCF7 and MDA-MB231, renal cancer cell line HEK-293T, and mesenchymal stem cell MSCs (Fig 1A). The gene discussed is IKZF2; the disease is breast carcinoma.