With regard to the leukemia-type short Helios isoforms, we found that Helios-V1 showed a predominant cytoplasmic localization (Fig 2C and 2D), whereas Helios-V2 and -V3, both of which lack exon 3–6, as well as all zinc-finger binding domains, exhibited both cytoplasmic and nuclear staining (Fig 2C and 2D). Here, IKZF2 is linked to leukemia.