HSPD1 and hepatocellular carcinoma: These results were further confirmed in HCC cell lines, As shown in Figure 5C, 5D and 5E Hsp60 overexpression significantly increased COX4 expression and mtDNA content when compared with control cells, while COX4 expression and mtDNA content were decreased when Hsp60 was silenced by siRNA (Figure 5C, 5D and 5E).