SPRY1 and cholangiocarcinoma: Comparing the differences in frequency distributions of alleles between cases and controls by χ2 test, we found there is a correlation between two loci (rs300574, SPRY1, OR = 1.312, 95% CI: 1.034–1.677, p = 0.026; rs1042725, HMGA2, OR = 1.309, 95% CI: 1.009–1.699, p = 0.043) and increased CC development under allele model.