ELAVL1 and colorectal carcinoma: To test the effects of MS-444 on HuR behavior in CRC cells, immunofluorescence and western blot analyses of HuR expression revealed that 10 μM MS-444 induced nuclear HuR localization in HCT116 cells with loss of cytoplasmic HuR occurring after 1 hr of treatment (Figure 3A-3B).