The same effect was observed with JMJD8 knockdown in HONE1 (Nasopharyngeal carcinoma cells), HaCat (Immortalized human keratinocytes) and U2OS (Osteosarcoma cells) cell lines indicating that the observed defects in NF-κB activation caused by JMJD8 knockdown is not cell-type specific (see Supplementary Fig. S2). This evidence concerns the gene JMJD8 and osteosarcoma.