CYCS and colorectal carcinoma: Our data demonstrate that DY and 5-FU dramatically triggered a release of cytochrome c from mitochondrial outer membrane in HCT-116 cells, what’s interesting, in RKO cells as well (Fig. 3c and Supplementary Fig. S4a), indicating that the combined treatment permeabilized mitochondrial outer membranes and may induce mitochondria-dependent apoptotic pathway in a synergistic way in two CRC cell lines, which was confirmed in the following work.