BMDM infection with S. Typhimurium (m.o.i. 10) in the presence of the caspase-8 inhibitor Z-IETD-FMK, as expected, had no effect on cell viability as measured by lactate dehydrogenase (LDH) activity (Fig. 6a) because caspase-8 does not induce pyroptosis in response to infection with this pathogen17. The gene discussed is CASP8; the disease is infection.