Of the 45 extreme ASE events observed in CHD probands (Supplementary Table 6), seven ASE events (Table 2) resulted from nonsense (ASPN, CTSA, PGM1 and RBFOX2), splice site (AARSD1) or frameshift (C7 and RETSAT) variants that caused NMD. Here, ASPN is linked to coronary artery disorder.