JAG1 and gastric cancer: We examined the effects of PFK15 on gastric cancer cells by trypan blue exclusion and observed a dose-dependent suppression of cell proliferation after 24 h on MKN45, AGS, and BGC823 gastric cancer cells (IC50: MKN45, 6.59±3.1 μmol/L; AGS, 8.54±2.7 μmol/L; BGC, 10.56±2.4 μmol/L) and a time-dependent inhibition over 48 h on MKN45 and AGS cells under a concentration of 10 μmol/L (Fig 1a and 1b).