As we know, BxPC-3 and Panc-1 cells have many differences such as K-ras mutation types (BxPC-3 is a K-ras wild cell while Panc-1 is a K-ras mutant cell) [34] and epithelial-mesenchymal transition (EMT) regulated genes (E-cadherin and Zeb-1) [35] which could affect gemcitabine sensitivity in pancreatic cancer cells. Here, KRAS is linked to familial pancreatic carcinoma.