EGFR and TGF-α are upregulated in pre-neoplastic FAH, HCA and HCC induced after NNM (Figure 1) administration and after PTX (Figure 2) and OTx (Figure 3) in untreated rat livers in comparison to extrafocal liver tissue, whereas TTx (Figure 4) induced lesions revealed no overexpression. The gene discussed is TGFA; the disease is hepatocellular carcinoma.