When the stratified data in subgroups of subjects by cigarette smoke status were analyzed, we found that smokers and non-smokers with mutated homozygous rs2181874 in CYP24A1 had significantly increased NSCLC risk (OR = 2.14, 95% CI 1.47–3.43; p = 0.031; OR = 3.57, 95% CI 2.66–4.74; p = 0.019, respectively). Here, CYP24A1 is linked to non-small cell lung carcinoma.