To further demonstrate the impact of DSCAM-AS1 on aggressive cancer phenotypes, we overexpressed DSCAM-AS1 in T47D (Supplementary Fig. 4c) and ZR75-1 (Supplementary Fig. 4d), two ER-positive breast cancer cell lines with moderate DSCAM-AS1 expression (Fig. 2c), and observed an increase in the invasion phenotype (Fig. 3g and Supplementary Fig. 4e). Here, DSCAM-AS1 is linked to cancer.