This approach nominated DSCAM-AS1 as a lncRNA expressed at a very high level in breast cancer tissues, containing ER promoter binding, and exhibiting the strongest oestrogen induction in MCF7 and T47D cells by both RNA-seq and quantitative PCR (qPCR) validation (Fig. 1c and Supplementary Fig. 2a). This evidence concerns the gene DSCAM-AS1 and breast cancer.