Univariate analysis (Table 6) showed that the age > 60 years (P = 0.022), AFP > 20 ng/mL (P = 0.018), liver cirrhosis (P < 0.001), HBV DNA > 2000 IU/mL (P = 0.04), and non-HCV SVR (P = 0.01) were higher in HCC patients compared to that in non-HCC patients. The gene discussed is AFP; the disease is cirrhosis of liver.