TWIST1 and nasopharyngeal carcinoma: After detecting Snail1 and Twist1 mRNAs in FlncKO-NPC embryos and their control littermates, we overlaid ISH images with IH images of β-Catenin, Cadherin, Tbr2 or Tuj1 to identify the relationship between cells with high Snail or Twist and those with aberrant adhesion or ectopic localization.