Distinct human BCa cell lines (from high malignancy to low malignancy: T24, 5637, EJ, UM-UC-3, BIU-87, RT-4) and immortalized normal uroepithelial cell line (SV-HUC-1) exhibited a downregulation tendency of OCT-4 by Western blot analysis (Figure 2B), suggesting OCT-4 could be a marker for bladder cancer. The gene discussed is POU5F1; the disease is urinary bladder cancer.