To determine the cellular mechanism that underlies the anti-GBM effect of FRMD6, we analyzed proliferation status of the transduced U87MG/U251 cells in vivo and observed that increased expression of FRMD6 inhibits the GBM cell proliferation as these GBM cells display the reduced Ki67 reactivity in situ comparing to the control GBM cells (Figure 3C). The gene discussed is FRMD6; the disease is glioblastoma.