We investigated the effect of FRMD6 knockdown on GBM cell proliferation and invasion and found that reduced FRMD6 expression significantly enhances GBM cell proliferation and invasion through Matrigel (Supplementary Figures S4 and S5), confirming that FRMD6 knockdown induces an effect opposite to that induced by increased FRMD6 expression. This evidence concerns the gene FRMD6 and glioblastoma.