In vitro binding assays were performed using purified recombinant GST-tagged-c-Cbl C- or N- terminus truncations with the lysates from different CRC cell lines, namely S33A phospho-resistant mutant β-catenin (HCT116), active β-catenin in the milieu of APC loss (HCT15) and wild-type β-catenin (RKO cells) (Supplementary Table 2). The gene discussed is CBL; the disease is colorectal carcinoma.