In this study, major findings were that HOTAIR was highly expressed in OS cell line MG-63 compared with normal osteoblast cell line hFOB1.19, the proliferation rate was lower, and the apoptosis rate was higher significantly when HOTAIR was knocked down in MG-63 cells, and tumor related factors TGF-β, p53, Bcl-2, and TNF-α were differently expressed in MG-63 cells when HOTAIR was knocked down. This evidence concerns the gene TP53 and neoplasm.