TGF-β is a member of TGF-β superfamily and mainly regulates the cell growth and differentiation processes which has been reported in various cancers. In vitro and in vivo studies have shown that TGF-β can regulate bone formation by inducing osteoblast and osteoclast proliferation [36] and high expression level was observed in osteosarcoma cells [37]. This evidence concerns the gene TGFB1 and osteosarcoma.