Moreover, we found that WT1 EP DCs from HLA-A*0201+ AML patient donors strongly stimulated IFN-γ secretion by the RHAMM R3-specific CD8+ cytotoxic T cell clone whereas those from HLA-A*0201− AML patient donors did not (mean 7.3 and 0.8 ng/105 T cells, respectively; p < 0.01; n = 3 and 2, respectively; Figure 5). This evidence concerns the gene CD8A and acute myeloid leukemia.