As shown in Fig. 2, knockdown of GGCT induced cellular senescence, as detected by SA-β-Gal staining, in MCF7 and MDA-MB-231 cells, as well as other cancer cell lines, including PC3 and LNCaP prostate cancer cells, HeLa cervical cancer cells, and A172 glioblastoma cells. Here, GGCT is linked to prostate cancer.