The present study proposes the concomitant analysis of 16 onco- and suppressor-genes (BRCA1, BRCA2, FOXO3, GATA4, HER2, HMGA1, HMGA2, HMGB1, MAPK1, MAPK3, MCL1, MYC, PFDN5, PIK3CA, PTEN, and TP53) regarded as being involved in neoplasm development using multiplex branched-DNA technology in fresh frozen (FF) and formalin-fixed, paraffin-embedded (FFPE) tissues. This evidence concerns the gene FOXO3 and neoplasm.