In order to test the role of Smyd2 in AML, we transduced Smyd2−/− and Smyd2+/+ hematopoietic stem and progenitor cells (HSPCs) with retroviruses expressing the MLL-AF9 and NRasG12D oncogenes, and transplanted the infected cells into sub-lethally irradiated syngeneic wild-type recipients. Here, SMYD2 is linked to acute myeloid leukemia.