IGF2BP1 and breast cancer: To confirm the negative effect of IMP1 transgene expression on pulmonary metastasis in MMTV-PyMT breast cancer mouse model, we performed both a one way anova analysis and Welch Two Sample t-test (P = 0.027), both analysis show that there is a statistical significance in the negative role of IMP1 transgene expression on the incidence of pulmonary metastasis between MMTV-PyMT-IMP1 mice (n = 7 mice, n = 7 lungs sections) expressing the IMP1 transgene and MMTV-PyMT-IMP1 mice (n = 7 mice, n = 7 lung sections) not expressing the IMP1 transgene (Figure 5).