To verify that the tumors expressed the atg5K130R transgene, we prepared short-term primary cell cultures of the tumors (to enrich for tumor cells and allow for depletion of nonproliferating normal cells), purified RNA from the cells and performed RT-PCR with primers specific for atg5. This assay validated expression of the atg5K130R transgene in the tumors (Supplementary Figure 1). This evidence concerns the gene ATG5 and neoplasm.